- Featured Product
- KD/KO Validated
LCN2 Polyclonal antibody
LCN2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 26991-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human saliva tissue, HT-29 cells, human saliva |
Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | RAW 264.7 cells, A431 cells |
Positive FC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 12 publications below |
IHC | See 8 publications below |
IF | See 5 publications below |
Product Information
26991-1-AP targets LCN2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LCN2 fusion protein Ag25715 |
Full Name | lipocalin 2 |
Observed molecular weight | 25 kDa |
Gene symbol | LCN2 |
Gene ID (NCBI) | 3934 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL, also known as human neutrophil lipocalin, lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL initially found in activated neutrophils, in accordance with its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond.
Protocols
Product Specific Protocols | |
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WB protocol for LCN2 antibody 26991-1-AP | Download protocol |
IHC protocol for LCN2 antibody 26991-1-AP | Download protocol |
IF protocol for LCN2 antibody 26991-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Neurol Neurosurg Psychiatry Molecules of senescent glial cells differentiate Alzheimer's disease from ageing | ||
Cell Chem Biol 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis. | ||
Oxid Med Cell Longev Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation.
| ||
Gastric Cancer Lipocalin-2 negatively regulates epithelial-mesenchymal transition through matrix metalloprotease-2 downregulation in gastric cancer.
| ||
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
iScience IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2 |