LCN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

LCN2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 26991-1-AP

Synonyms

24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25

---

Validation Data Gallery

• 

  WB analysis of human saliva using 26991-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 26991-1-AP (LCN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26991-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 26991-1-AP (LCN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 26991-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LCN2 antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of RAW 264.7 using 26991-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using LCN2 antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of A431 using 26991-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human LCN2 (26991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: human saliva tissue, HT-29 cells, human saliva
• Positive IHC detected in: human colon cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: RAW 264.7 cells, A431 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 8 publications below
• IF: See 5 publications below

Product Information

26991-1-AP targets LCN2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LCN2 fusion protein Ag25715
• Full Name: lipocalin 2
• Observed molecular weight: 25 kDa
• Gene symbol: LCN2
• Gene ID (NCBI): 3934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neutrophil gelatinase-associated lipocalin (NGAL, also known as human neutrophil lipocalin, lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL initially found in activated neutrophils, in accordance with its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond.

Publications

Species	Application	Title
human	WB	J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
	WB	Cell Chem Biol; 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.; Authors - Chenwen Shao
human	WB	Oxid Med Cell Longev; Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation.; Authors - Su Zhao ; KD Validated
human	WB,IHC	Gastric Cancer; Lipocalin-2 negatively regulates epithelial-mesenchymal transition through matrix metalloprotease-2 downregulation in gastric cancer.; Authors - Sadaaki Nishimura ; KD Validated
human	IF	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	iScience; IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2; Authors - Yufei Lan