• Featured Product
  • KD/KO Validated

LCN2 Polyclonal antibody

LCN2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26991-1-AP

Synonyms

24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25



Tested Applications

Positive WB detected inhuman saliva tissue, HT-29 cells, human saliva
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells, A431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

26991-1-AP targets LCN2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LCN2 fusion protein Ag25715
Full Name lipocalin 2
Observed molecular weight 25 kDa
Gene symbol LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neutrophil gelatinase-associated lipocalin (NGAL, also known as human neutrophil lipocalin, lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL initially found in activated neutrophils, in accordance with its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond.

Protocols

Product Specific Protocols
WB protocol for LCN2 antibody 26991-1-APDownload protocol
IHC protocol for LCN2 antibody 26991-1-APDownload protocol
IF protocol for LCN2 antibody 26991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neurol Neurosurg Psychiatry

Molecules of senescent glial cells differentiate Alzheimer's disease from ageing

Authors - Linbin Dai
WB

Cell Chem Biol

3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.

Authors - Chenwen Shao
humanWB

Oxid Med Cell Longev

Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation.

Authors - Su Zhao
  • KD Validated
humanWB,IHC

Gastric Cancer

Lipocalin-2 negatively regulates epithelial-mesenchymal transition through matrix metalloprotease-2 downregulation in gastric cancer.

Authors - Sadaaki Nishimura
  • KD Validated
humanIF

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
humanWB

iScience

IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2

Authors - Yufei Lan