LDAH Polyclonal antibody

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  WB analysis using 31714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31714-1-AP (C2orf43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 31714-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31714-1-AP (LDAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LDAH Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31714-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C2orf43, UPF0554 protein C2orf43, Lipid Droplet Associated Hydrolase, HLDAH

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31714-1-AP targets LDAH in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36372

  Product name: Recombinant human C2orf43 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-325 aa of BC017473

  Sequence: DSNAQEIKDIYGLNGQIEHKLAFLRTHVPKDMKLVLIGHSIGSYFTLQMLKRVPELPVIRAFLLFPTIERMSESPNGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRRGLQVMNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDDETIKEHLCKLTFYYGTIDPWCPKEYYEDIKKDFPEGDIRLCEKNIPHAFITHFNQEMADMIADSLKDDLSKM

• Full Name: chromosome 2 open reading frame 43
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC017473
• Gene Symbol: C2orf43
• Gene ID (NCBI): 60526
• RRID: AB_3670093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H6V9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDAH, also known as C2orf43, is a newly identified LD protein. It has 4 isoforms and is abundantly expressed in tissues that predominantly store triacylglycerol (TAG), including liver and white and brown adipose tissue (PMID: 24357060).