LDB2 Polyclonal antibody

LDB2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11873-1-AP

Synonyms

CLIM 1, CLIM1, LDB 2, LDB1, LDB2, LIM domain binding 2, LIM domain binding protein 2

---

Validation Data Gallery

• 

  IHC staining of rat brain using 11873-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11873-1-AP (LDB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11873-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11873-1-AP (LDB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11873-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11873-1-AP (LDB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11873-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11873-1-AP (LDB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

11873-1-AP targets LDB2 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDB2 fusion protein Ag2460
• Full Name: LIM domain binding 2
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: BC034019
• Gene Symbol: LDB2
• Gene ID (NCBI): 9079
• RRID: AB_2833068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nature; Genetic dissection of the glutamatergic neuron system in cerebral cortex.; Authors - Katherine S Matho
human	IF	Circ Heart Fail; Overexpression of ATP5F1A in Cardiomyocytes Promotes Cardiac Reverse Remodeling; Authors - Mengda Xu