LDB3 Polyclonal antibody

LDB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11004-1-AP

Synonyms

CYPHER, KIAA01613, KIAA0613, LDB3, ldb3z1, ldb3z4, LIM domain binding 3, LIM domain binding protein 3, ORACLE, PDLIM6, Protein cypher, ZASP

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 11004-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11004-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11004-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 11004-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11004-1-AP (LDB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human skeletal muscle tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11004-1-AP targets LDB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDB3 fusion protein Ag1417
• Full Name: LIM domain binding 3
• Calculated Molecular Weight: 32/78 kDa
• Observed Molecular Weight: 32 kDa, 78-80 kDa
• GenBank Accession Number: BC010929
• Gene Symbol: LDB3
• Gene ID (NCBI): 11155
• RRID: AB_2137049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; α-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilisation.; Authors - Garton F F
mouse, human	WB	Hum Mol Genet; Analysis of the ACTN3 Heterozygous Genotype Suggests That α-Actinin-3 Controls Sarcomeric Composition and Muscle Function in a Dose-Dependent Fashion.; Authors - Marshall W Hogarth
human	IHC	NPJ Precis Oncol; Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling; Authors - Shinji Kohsaka