LDB3 Polyclonal antibody

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• LDB3 Polyclonal antibody (11004-1-AP)

LDB3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11004-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

CYPHER, KIAA01613, KIAA0613, LDB3, ldb3z1, ldb3z4, LIM domain binding 3, LIM domain binding protein 3, ORACLE, PDLIM6, Protein cypher, ZASP

• 

  WB analysis of mouse skeletal muscle using 11004-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human skeletal muscle using 11004-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 11004-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 11004-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11004-1-AP (LDB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human skeletal muscle tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11004-1-AP targets LDB3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1417

  Product name: Recombinant human LDB3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-283 aa of BC010929

  Sequence: MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIKSASYNLSLTLQKSKRPIPISTTAPPVQTPLPVIPHQKVVVNSPANADYQERFNPSALKDSALSTHKPIEVKGLGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGSDFSGSLPIKDLAVDSASPVYQAVIKSQNKPEDEADEWARRSSNLQSRSFRILAQMTGTEFMQDPDEEALRRSRERFETERNSPRFAKLRNWHHGLSAQILNVKS

• Full Name: LIM domain binding 3
• Calculated Molecular Weight: 32/78 kDa
• Observed Molecular Weight: 32 kDa, 78-80 kDa
• GenBank Accession Number: BC010929
• Gene Symbol: LDB3
• Gene ID (NCBI): 11155
• RRID: AB_2137049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75112
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-ACTN3 (α-Actinin-3) antibody, catalog number 11004-1-AP, has 4 total citations. These appear in NPJ Precision Oncology, Journal of Cellular and Molecular Medicine, and Human Molecular Genetics. Research fields span salivary duct carcinoma biomarker discovery, aortic dissection hub gene identification, and skeletal muscle adaptation related to ACTN3 genotype effects on sarcomeric composition and function.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	NPJ Precis Oncol; Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling; Authors - Shinji Kohsaka
human,mouse	WB,IF	J Cell Mol Med; Identification of the Hub Gene LDB3 in Stanford Type A Aortic Dissection Based on Comprehensive Bioinformatics Analysis; Authors - Xinyi Liu
mouse, human	WB	Hum Mol Genet; Analysis of the ACTN3 Heterozygous Genotype Suggests That α-Actinin-3 Controls Sarcomeric Composition and Muscle Function in a Dose-Dependent Fashion.; Authors - Marshall W Hogarth
mouse	WB	Hum Mol Genet; α-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilisation.; Authors - Garton F F