LDB3 Polyclonal antibody

LDB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11004-1-AP

Synonyms

CYPHER, KIAA01613, KIAA0613, LDB3, ldb3z1, ldb3z4, LIM domain binding 3, LIM domain binding protein 3, ORACLE, PDLIM6, Protein cypher, ZASP



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11004-1-AP targets LDB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDB3 fusion protein Ag1417
Full Name LIM domain binding 3
Calculated Molecular Weight 32/78 kDa
Observed Molecular Weight 32 kDa, 78-80 kDa
GenBank Accession NumberBC010929
Gene Symbol LDB3
Gene ID (NCBI) 11155
RRIDAB_2137049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LDB3 antibody 11004-1-APDownload protocol
IHC protocol for LDB3 antibody 11004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

α-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilisation.

Authors - Garton F F
mouse, humanWB

Hum Mol Genet

Analysis of the ACTN3 Heterozygous Genotype Suggests That α-Actinin-3 Controls Sarcomeric Composition and Muscle Function in a Dose-Dependent Fashion.

Authors - Marshall W Hogarth
humanIHC

NPJ Precis Oncol

Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling

Authors - Shinji Kohsaka