LDHA-Specific Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 84198-3-RR (same clone as 84198-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84198-3-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84198-3-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-231 using 84198-3-RR (same clone as 84198-3-PBS)

  WB result of LDHA-Specific antibody (84198-3-RR; 1:1000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP03165-1

  The mean LDHA concentration was determined to be 61.9 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 6.4 ng/mL in U-251 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP03165-1

  Sandwich ELISA standard curve of MP03165-1, Human LDHA Recombinant Matched Antibody Pair - PBS only. 84198-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00607. 84198-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  FC experiment of HepG2 using 84198-3-RR (same clone as 84198-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-3-RR, Clone:240885C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84198-3-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LDHA-Specific Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA

Cat No. 84198-3-PBS

Clone No.240885C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA

Synonyms

LDHA, 240885C7, Cell proliferation-inducing gene 19 protein, EC:1.1.1.27, lactate dehydrogenase A

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Product Information

84198-3-PBS targets LDHA-Specific as part of a matched antibody pair:

MP03165-1: 84198-3-PBS capture and 84198-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: lactate dehydrogenase A
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_005566
• Gene Symbol: LDHA
• Gene ID (NCBI): 3939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00338
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.