LDHB Polyclonal antibody

• Featured Product
• KD/KO Validated

LDHB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

59

Cat no : 14824-1-AP

Synonyms

lactate dehydrogenase B, EC:1.1.1.27, LDHA, LDH H, LDH B

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Validation Data Gallery

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  WB analysis using 14824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14824-1-AP (LDHB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14824-1-AP

  WB result of LDHB antibody (14824-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDHB transfected HeLa cells.

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  IP experiment of mouse brain using 14824-1-AP

  IP result of anti-LDHB (IP:14824-1-AP, 4ug; Detection:14824-1-AP 1:20000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of human kidney using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14824-1-AP (LDHB antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human kidney using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14824-1-AP (LDHB antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human prostate cancer using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14824-1-AP (LDHB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14824-1-AP (LDHB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14824-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDHB antibody (14824-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14824-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDHB antibody (14824-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, DU 145 cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 50 publications below
• IHC: See 8 publications below
• IF: See 8 publications below

Product Information

14824-1-AP targets LDHB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDHB fusion protein Ag6605
• Full Name: lactate dehydrogenase B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002362
• Gene Symbol: LDHB
• Gene ID (NCBI): 3945
• RRID: AB_2134953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway. LDH comprises LDHA and LDHB, two subunits that are encoded by independent genes. In muscle or liver, most of the LDH is composed of four LDHA subunits, and preferably catalyzes the reduction of pyruvate to lactate. In heart and brain, LDH is mainly composed of four LDHB subunits, and predominantly catalyzes the oxidation of lactate to pyruvate. In other tissues, LDH is composed of both LDHA and LDHB. (PMID: 26269128, 31804482)

Publications

Species	Application	Title
human	WB	Nat Methods; A large-scale targeted proteomics assay resource based on an in vitro human proteome.; Authors - Masaki Matsumoto
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Nucleic Acids Res; Histone lactylation-boosted ALKBH3 potentiates tumor progression and diminished promyelocytic leukemia protein nuclear condensates by m1A demethylation of SP100A; Authors - Xiang Gu
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
mouse	WB	Nat Commun; LDHA-mediated ROS generation in chondrocytes is a potential therapeutic target for osteoarthritis.; Authors - Manoj Arra