LEF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LEF1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

44

Cat no : 14972-1-AP

Synonyms

LEF 1, LEF1, TCF1 alpha, TCF1ALPHA

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Validation Data Gallery

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  WB analysis using 14972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14972-1-AP (LEF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14972-1-AP

  WB result of LEF1 antibody (14972-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-LEF1 transfected Jurkat cells.

• 

  WB analysis of COLO 320 using 14972-1-AP

  WB result of LEF1 antibody (14972-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LEF1 transfected COLO 320 cells.

• 

  WB analysis using 14972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14972-1-AP (LEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SW 1990 using 14972-1-AP

  IP result of anti-LEF1 (IP:14972-1-AP, 4ug; Detection:14972-1-AP 1:300) with SW 1990 cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: Jurkat cells, COLO 320 cells, NCCIT cells
• Positive IP detected in: SW 1990 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 39 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

14972-1-AP targets LEF1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LEF1 fusion protein Ag6882
• Full Name: lymphoid enhancer-binding factor 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 50-55 kDa, 65 kDa
• GenBank Accession Number: BC050632
• Gene Symbol: LEF1
• Gene ID (NCBI): 51176
• RRID: AB_2265677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphoid enhancer-binding factor 1(LEF1) belongs to a family of regulatory proteins that share homology with high mobility group protein-1, and it's a nuclear protein expressed in pre-B and T cells. LEF1 has a role in the Wnt signaling pathway hair cell differentiation and follicle morphogenesis. Together with CTNNB1 and EP300, LEF1 activates transcription of target genes. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances the proliferation of pancreatic tumor cells. MECs can give rise to seven cell types of the SAE and SMGs following severe airway injury. MECs progressively adopted a basal cell phenotype on the SAE and established lasting progenitors capable of further regeneration following reinjury. MECs activate Wnt-regulated transcription factors (Lef-1/TCF7) following injury and Lef-1 induction in cultured MECs promotes transition to a basal cell phenotype. Surprisingly, dose-dependent MEC conditional activation of Lef-1in vivopromoted self-limited airway regeneration in the absence of injury. Thus, modulating the Lef-1 transcriptional program in MEC-derived progenitors may have regenerative medicine applications for lung diseases. (https://doi.org/10.1016/j.stem.2018.03.017) The phosphorylation may affect the LEF1 protein's theoretical molecular weight when tested.40-70 kD bands have also been reported (PMID: 22261717; 17063141 ).

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
mouse	WB,IF	J Allergy Clin Immunol; Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.; Authors - Yukai Jing
mouse	WB	Cell Death Differ; MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.; Authors - Lifang Hu
human	WB	Clin Cancer Res; microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma.; Authors - Xiao-Yan He
human	WB	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu ; KD Validated
mouse		Cell Death Dis; Inhibition of phosphodiesterase 5 reduces bone mass by suppression of canonical Wnt signaling.; Authors - Y Gong