LEMD2 Polyclonal antibody

LEMD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29406-1-AP

Synonyms

dJ482C21.1, hLEM2, LEM domain containing 2, LEMD2

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Validation Data Gallery

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  WB analysis using 29406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29406-1-AP (LEMD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 29406-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29406-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 29406-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 29406-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells, human testis tissue
• Positive IHC detected in: human colon tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29406-1-AP targets LEMD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LEMD2 fusion protein Ag28078
• Full Name: LEM domain containing 2
• Calculated Molecular Weight: 503 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC051803
• Gene Symbol: LEMD2
• Gene ID (NCBI): 221496
• RRID: AB_2918298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.