LENG8 Polyclonal antibody

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  WB analysis of A2780 using 13336-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 13336-1-AP (LENG8 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 13336-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13336-1-AP (LENG8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 13336-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug LENG8 Polyclonal antibody (13336-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

LENG8 Polyclonal Antibody for WB, FC (Intra), ELISA

Cat No. 13336-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

pp13842, KIAA1932

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Tested Applications

• Positive WB detected in: A2780 cells, Jurkat cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13336-1-AP targets LENG8 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4153

  Product name: Recombinant human LENG8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 501-800 aa of BC028048

  Sequence: EDPERELKKQKRAARFQHGHSRRLRLEPLVLQMSSLESSGADPDWQELQIVGTCPDITKHYLRLTCAPDPSTVRPVAVLKKSLCMVKCHWKEKQDYAFACEQMKSIRQDLTVQGIRTEFTVEVYETHARIALEKGDHEEFNQCQTQLKSLYAENLPGNVGEFTAYRILYYIFTKNSGDITTELAYLTRELKADPCVAHALALRTAWALGNYHRFFRLYCHAPCMSGYLVDKFADRERKVALKAMIKTFRPALPVSYLQAELAFEGEAACRAFLEPLGLAYTGPDNSSIDCRLSLAQLSAF

• Full Name: leukocyte receptor cluster (LRC) member 8
• Calculated Molecular Weight: 800 aa, 88 kDa
• Observed Molecular Weight: 86-88 kDa
• GenBank Accession Number: BC028048
• Gene Symbol: LENG8
• Gene ID (NCBI): 114823
• RRID: AB_1167573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PV6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Clin Exp Pathol; Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.; Authors - Xiaojuan Ye