LEO1 Polyclonal antibody

LEO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12281-1-AP

Synonyms

RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, RDL

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Validation Data Gallery

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  WB analysis of mouse heart using 12281-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12281-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12281-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12281-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12281-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12281-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12281-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 12281-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12281-1-AP (LEO1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12281-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12281-1-AP (LEO1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12281-1-AP

  Immunofluorescent analysis of Hela cells, using LEO1 antibody 12281-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, HL-60 cells, human placenta tissue, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

12281-1-AP targets LEO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LEO1 fusion protein Ag2932
• Full Name: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 666 aa, 75 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC018147
• Gene Symbol: LEO1
• Gene ID (NCBI): 123169
• RRID: AB_10640429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).

Publications

Species	Application	Title
human	WB	Nat Neurosci; Toxic expanded GGGGCC repeat transcription is mediated by the PAF1 complex in C9orf72-associated FTD.; Authors - Lindsey D Goodman
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
human	WB	Sci Adv; CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation; Authors - Min Qiu
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao