LEO1 Polyclonal antibody

LEO1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12281-1-AP

Synonyms

LEO1, RDL



Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, HL-60 cells, human placenta tissue, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12281-1-AP targets LEO1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LEO1 fusion protein Ag2932
Full Name Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight 666 aa, 75 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC018147
Gene symbol LEO1
Gene ID (NCBI) 123169
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).

Protocols

Product Specific Protocols
WB protocol for LEO1 antibody 12281-1-APDownload protocol
IHC protocol for LEO1 antibody 12281-1-APDownload protocol
IF protocol for LEO1 antibody 12281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Neurosci

Toxic expanded GGGGCC repeat transcription is mediated by the PAF1 complex in C9orf72-associated FTD.

Authors - Lindsey D Goodman