LEPR Polyclonal antibody

LEPR Polyclonal Antibody for WB, ELISA

Cat No. 32817-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF, ELISA

Synonyms

B219, CD295, Db, LEP-R, Leptin receptor

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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WB analysis using 32817-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 32817-1-AP (LEPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for LEPR in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

mouse heart tissue, rat heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32817-1-AP targets LEPR in WB, IF, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity	mouse, rat
Cited Reactivity	rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Eg2930 Product name: Recombinant Mouse Lepr protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 22-839 aa of NM_146146.2 Sequence: LNLAYPISPWKFKLFCGPPNTTDDSFLSPAGAPNNASALKGASEAIVEAKFNSSGIYVPELSKTVFHCCFGNEQGQNCSALTDNTEGKTLASVVKASVFRQLGVNWDIECWMKGDLTLFICHMEPLPKNPFKNYDSKVHLLYDLPEVIDDSPLPPLKDSFQTVQCNCSLRGCECHVPVPRAKLNYALLMYLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLHMEVTDDGNLKISWDSQTMAPFPLQYQVKYLENSTIVREAAEIVSATSLLVDSVLPGSSYEVQVRSKRLDGSGVWSDWSSPQVFTTQDVVYFPPKILTSVGSNASFHCIYKNENQIISSKQIVWWRNLAEKIPEIQYSIVSDRVSKVTFSNLKATRPRGKFTYDAVYCCNEQACHHRYAELYVIDVNINISCETDGYLTKMTCRWSPSTIQSLVGSTVQLRYHRRSLYCPDSPSIHPTSEPKNCVLQRDGFYECVFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITVNTGLLKVSWEKPVFPENNLQFQIRYGLSGKEIQWKTHEVFDAKSKSASLLVSDLCAVYVVQVRCRRLDGLGYWSNWSSPAYTLVMDVKVPMRGPEFWRKMDGDVTKKERNVTLLWKPLTKNDSLCSVRRYVVKHRTAHNGTWSEDVGNRTNLTFLWTEPAHTVTVLAVNSLGASLVNFNLTFSWPMSKVSAVESLSAYPLSSSCVILSWTLSPDDYSLLYLVIEWKILNEDDGMKWLRIPSNVKKFYIHDNFIPIEKYQFSLYPVFMEGVGKPKIINGFTKDAIDKQQNDAG Predict reactive species
Full Name	leptin receptor
Calculated Molecular Weight	131 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	NM_146146.2
Gene Symbol	Lepr
Gene ID (NCBI)	16847
RRID	AB_3742753
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	P48356-1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leptin receptor (LEPR) is a protein-coding gene that encodes a receptor for leptin, an adipocyte-specific hormone that regulates body weight and fat metabolism (PMID: 11861497; 16333525). LEPR is involved in various physiological processes, including energy homeostasis, immune function, and hematopoiesis (PMID: 11923481; 25383904). It activates the JAK2/STAT3 signaling pathway, which is crucial for mediating leptin's effects on gene transcription and cellular responses (PMID: 11923481). LEPR also plays a role in the regulation of lipid metabolism, blood pressure, and blood glucose levels (PMID: 29254602).

Protocols

Product Specific Protocols
WB protocol for LEPR antibody 32817-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The LEPR polyclonal antibody (Cat# 32817-1-AP) has 1 citation to date. The cited publication appeared in Nature Communications (IF 18.1, 2025) and investigated a directed rescue strategy for enhanced implant osteointegration in aged rats, using the antibody for WB and IF applications in rat samples. The research field encompasses bone biology, implant integration, and aging-related tissue regeneration.