LETM1 Monoclonal antibody, PBS Only (Detector)

• Featured Product
• KD/KO Validated

LETM1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B9B9

Cat no : 68208-1-PBS

Synonyms

Leucine zipper-EF-hand-containing transmembrane protein 1, 2B9B9

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Validation Data Gallery

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  WB analysis using 68208-1-Ig (same clone as 68208-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 68208-1-Ig (same clone as 68208-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 68208-1-Ig (same clone as 68208-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68208-1-Ig (same clone as 68208-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68208-1-Ig (same clone as 68208-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50653-2

  Cytometric bead array standard curve of MP50653-2, LETM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68208-4-PBS. Detection antibody: 68208-1-PBS. Standard:Ag8851. Range: 0.391-100 ng/mL.

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Product Information

68208-1-PBS targets LETM1 as part of a matched antibody pair:

MP50653-2: 68208-4-PBS capture and 68208-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LETM1 fusion protein Ag8851
• Full Name: leucine zipper-EF-hand containing transmembrane protein 1
• Calculated Molecular Weight: 739 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC021208
• Gene Symbol: LETM1
• Gene ID (NCBI): 3954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.