CD11a/Integrin Alpha L Polyclonal antibody

CD11a/Integrin Alpha L Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15574-1-AP

Synonyms

CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15574-1-AP targets CD11a/Integrin Alpha L in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD11a/Integrin Alpha L fusion protein Ag7920
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated Molecular Weight 129 kDa
GenBank Accession NumberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
RRIDAB_2878152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
IHC protocol for CD11a/Integrin Alpha L antibody 15574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Front Immunol

ITGAL expression in non-small-cell lung cancer tissue and its association with immune infiltrates

Authors - Ruihao Zhang
humanWB

J Neuroinflammation

Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.

Authors - Ceshu Gao
humanIHC

Transl Oncol

Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level.

Authors - Xuyong Wei