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Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
15574-1-AP targets CD11a/Integrin Alpha L in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD11a/Integrin Alpha L fusion protein Ag7920 Predict reactive species |
| Full Name | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
| Calculated Molecular Weight | 129 kDa |
| GenBank Accession Number | BC008777 |
| Gene Symbol | CD11a |
| Gene ID (NCBI) | 3683 |
| ENSEMBL Gene ID | ENSG00000005844 |
| RRID | AB_2878152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20701 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD11a/Integrin Alpha L antibody 15574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol ITGAL expression in non-small-cell lung cancer tissue and its association with immune infiltrates | ||
J Neuroinflammation Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke. | ||
Transl Oncol Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level. |
