Galectin 2 Polyclonal antibody

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  WB analysis using 12849-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12849-2-AP (Galectin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12849-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12849-2-AP (Galectin 2 antibody) at dilution of 1:100 (under 10x lens).

Galectin 2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12849-2-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Gal 2, Galectin 2, HL14, Lactose binding lectin 2, LGALS2, S Lac lectin 2

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, rat small intestine tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

12849-2-AP targets Galectin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galectin 2 fusion protein Ag3979
• Full Name: lectin, galactoside-binding, soluble, 2
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC029063
• Gene Symbol: Galectin 2
• Gene ID (NCBI): 3957
• RRID: AB_2136753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05162
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncotarget; Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.; Authors - Maria Claudia Vladoiu
human	WB,IHC	J Gastroenterol; Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer.; Authors - Jung Ji-Han JH
human	IHC	Oncotarget; Galectin signatures contribute to the heterogeneity of breast cancer and provide new prognostic information and therapeutic targets.; Authors - Andrée-Anne Grosset