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LGALS3BP Polyclonal antibody

LGALS3BP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23307-1-AP

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using LGALS3BP Polyclonal antibody (23307-1-AP)

    WB analysis of HepG2 using 23307-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    WB analysis of mouse brain using 23307-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human skin using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LGALS3BP Polyclonal antibody (23307-1-AP)

    IF Staining of HepG2 using 23307-1-AP

    Immunofluorescent analysis of HepG2 cells using 23307-1-AP (LGALS3BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23307-1-AP is LGALS3BP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGALS3BP fusion protein Ag19700
Full Name lectin, galactoside-binding, soluble, 3 binding protein
Calculated Molecular Weight 585 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC002403
Gene Symbol LGALS3BP
Gene ID (NCBI) 3959
RRIDAB_2879251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Protocols

Product Specific Protocols
WB protocol for LGALS3BP antibody 23307-1-APDownload protocol
IHC protocol for LGALS3BP antibody 23307-1-APDownload protocol
IF protocol for LGALS3BP antibody 23307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols