LGALS9/Galectin-9 Polyclonal antibody

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  WB analysis using 30409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30409-1-AP (LGALS9/Galectin-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

LGALS9/Galectin-9 Polyclonal Antibody for WB, ELISA

Cat No. 30409-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Galectin 9, LGALS9, Ecalectin, Gal 9, Gal-9

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Tested Applications

• Positive WB detected in: THP-1 cells, U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30409-1-AP targets LGALS9/Galectin-9 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGALS9/Galectin-9 fusion protein Eg0084
• Full Name: lectin, galactoside-binding, soluble, 9
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC110340
• Gene Symbol: Galectin-9
• Gene ID (NCBI): 3965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00182
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.