LHPP Monoclonal antibody

LHPP Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Mouse, Rat, Pig, human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A2A4

Cat no : 66988-1-Ig

Synonyms

hLHPP, LHPP

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Validation Data Gallery

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  WB analysis of rat liver using 66988-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66988-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66988-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66988-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66988-1-Ig (LHPP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat liver tissue, pig liver tissue, mouse brain tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66988-1-Ig targets LHPP in WB, IF, ELISA applications and shows reactivity with Mouse, Rat, Pig, human samples.

• Tested Reactivity: Mouse, Rat, Pig, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LHPP fusion protein Ag8375
• Full Name: phospholysine phosphohistidine inorganic pyrophosphate phosphatase
• Calculated Molecular Weight: 23 kDa, 29 kDa
• Observed Molecular Weight: 23-30 kDa
• GenBank Accession Number: BC007324
• Gene Symbol: LHPP
• Gene ID (NCBI): 64077
• RRID: AB_2882305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.