LHPP Monoclonal antibody

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  WB analysis of rat liver using 66988-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66988-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66988-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66988-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LHPP antibody (66988-1-Ig, Clone: 2A2A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

LHPP Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66988-1-Ig

Clone No.2A2A4

Host / Isotype

Mouse / IgG1

Reactivity

Mouse, Rat, Pig, human

Applications

WB, IF/ICC, ELISA

Synonyms

hLHPP, LHPP

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Tested Applications

• Positive WB detected in: rat liver tissue, pig liver tissue, mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66988-1-Ig targets LHPP in WB, IF/ICC, ELISA applications and shows reactivity with Mouse, Rat, Pig, human samples.

• Tested Reactivity: Mouse, Rat, Pig, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8375

  Product name: Recombinant human LHPP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-210 aa of BC007324

  Sequence: MAPWGKRLAGVRGVLLDISGVLYDSGAGGGTAIAGSVEAVARLKRSRLKVRFCTNESQKSRAELVGQLQRLGFDISEQEVTAPAPAACQILKERGLRPYLLIHDGVRSEFDQIDTSNPNCVVIADAGESFSYQNMNNAFQVLMELEKPVLISLGKGRYYKETSGLMLDVGPYMKALEYACGIKAEVVGKPSPEFFKSALQAIGVEAHQAQ

• Full Name: phospholysine phosphohistidine inorganic pyrophosphate phosphatase
• Calculated Molecular Weight: 23 kDa, 29 kDa
• Observed Molecular Weight: 23-30 kDa
• GenBank Accession Number: BC007324
• Gene Symbol: LHPP
• Gene ID (NCBI): 64077
• RRID: AB_2882305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9H008
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.