LHX4 Polyclonal antibody

LHX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11183-1-AP

Synonyms

Gsh 4, Gsh4, LHX4, LIM homeobox 4, LIM homeobox protein 4, LIM/homeobox protein Lhx4

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Validation Data Gallery

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  WB analysis of HepG2 using 11183-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11183-1-AP (LHX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

11183-1-AP targets LHX4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, monkey, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LHX4 fusion protein Ag1642
• Full Name: LIM homeobox 4
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-47 kDa
• GenBank Accession Number: BC011759
• Gene Symbol: LHX4
• Gene ID (NCBI): 89884
• RRID: AB_2858194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
chicken	IHC	Sci Rep; Notch signaling represses cone photoreceptor formation through the regulation of retinal progenitor cell states.; Authors - Xueqing Chen
mouse	IF	Invest Ophthalmol Vis Sci; Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors.; Authors - Diego F Buenaventura
mouse	WB	Cell Rep; LIM-Homeodomain Transcription Factor LHX4 Is Required for the Differentiation of Retinal Rod Bipolar Cells and OFF-Cone Bipolar Subtypes.; Authors - Xuhui Dong
mouse	IF	Glia; NFkB-signaling promotes glial reactivity and suppresses Müller glia-mediated neuron regeneration in the mammalian retina; Authors - Isabella Palazzo
monkey	IHC	Differentiation; Expression patterns of CYP26A1, FGF8, CDKN1A, and NPVF in the developing rhesus monkey retina; Authors - Miranda R Krueger
mouse	IF	Cell Mol Life Sci; Lhx4 surpasses its paralog Lhx3 in promoting the differentiation of spinal V2a interneurons; Authors - Estelle Renaux