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LHX4 Polyclonal antibody

LHX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Gsh 4, Gsh4, LIM homeobox 4, LIM homeobox protein 4, LIM/homeobox protein Lhx4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHepG2 cells, mouse lung tissue, rat lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11183-1-AP targets LHX4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, monkey, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1642

Product name: Recombinant human LHX4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-390 aa of BC011759

Sequence: MMQSATVPAEGAVKGLPEMLGVPMQQIPQCAGCNQHILDKFILKVLDRHWHSSCLKCADCQMQLADRCFSRAGSVYCKEDFFKRFGTKCTACQQGIPPTQVVRKAQDFVYHLHCFACIICNRQLATGDEFYLMEDGRLVCKEDYETAKQNDDSEAGAKRPRTTITAKQLETLKNAYKNSPKPARHVREQLSSETGLDMRVVQVWFQNRRAKEKRLKKDAGRHRWGQFYKSVKRSRGSSKQEKESSAEDCGVSDSELSFREDQILSELGHTNRIYGNVGDVTGGQLMNGSFSMDGTGQSYQDLRDGSPYGIPQSPSSISSLPSHAPLLNGLDYTVDSNLGIIAHAGQGVSQTLRAMAGGPTSDISTGSSVGYPDFPTSPGSWLDEMDHPPF

Predict reactive species
Full Name LIM homeobox 4
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43-47 kDa
GenBank Accession NumberBC011759
Gene Symbol LHX4
Gene ID (NCBI) 89884
RRIDAB_2858194
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969G2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LHX4 antibody 11183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The LHX4 Polyclonal antibody (Cat# 11183-1-AP) has 7 citations published in Cell Reports, Cell & Molecular Life Sciences, Investigative Ophthalmology & Visual Science, Glia, Scientific Reports, Journal of Neuroscience, and Differentiation. The research fields span retinal development (rod bipolar cell and cone photoreceptor differentiation), spinal interneuron specification, glial reactivity, Müller glia-mediated neuroprotection, and neuronal regeneration in the mammalian retina.

SpeciesApplicationTitle
mouseWB

Cell Rep

LIM-Homeodomain Transcription Factor LHX4 Is Required for the Differentiation of Retinal Rod Bipolar Cells and OFF-Cone Bipolar Subtypes.

Authors - Xuhui Dong
mouseIF

Cell Mol Life Sci

Lhx4 surpasses its paralog Lhx3 in promoting the differentiation of spinal V2a interneurons

Authors - Estelle Renaux
mouseIF

Invest Ophthalmol Vis Sci

Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors.

Authors - Diego F Buenaventura
mouseIF

Glia

NFkB-signaling promotes glial reactivity and suppresses Müller glia-mediated neuron regeneration in the mammalian retina

Authors - Isabella Palazzo
chickenIHC

Sci Rep

Notch signaling represses cone photoreceptor formation through the regulation of retinal progenitor cell states.

Authors - Xueqing Chen
mouseIF

J Neurosci

Sphingosine-1-Phosphate Signaling through Müller Glia Regulates Neuroprotection, Accumulation of Immune Cells, and Neuronal Regeneration in the Rodent Retina.

Authors - Olivia Taylor
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