LILRA5/CD85f Polyclonal antibody

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  WB analysis of K-562 using 18821-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18821-1-AP (LILRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18821-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18821-1-AP (LILRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

LILRA5/CD85f Polyclonal Antibody for WB, ELISA

Cat No. 18821-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LILRA5, CD85, CD85 antigen-like family member F, CD85f, ILT 11

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18821-1-AP targets LILRA5/CD85f in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 31-33 kDa
• GenBank Accession Number: BC153203
• Gene Symbol: LILRA5
• Gene ID (NCBI): 353514
• RRID: AB_2281279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A6NI73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LILRA5, also named as CD85f, ILT11, LILRB7 or LIR9, is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is a group of immune inhibitory and stimulatory receptors (PMID: 15304001). There are two membrane-bound and two soluble forms of LILRA5. The transmembrane LILRA5 contains a short cytoplasmic domain and a charged arginine residue within the transmembrane region (PMID: 19009525). It is mostly expressed in myeloid cells, including monocytes and neutrophils (PMID: 12393390). LILRA5 may play a role in triggering innate immune responses. It does not function in class I MHC antigens recognition (PMID: 16675463). The antibody recognizes the isoform 1 and isoform 2 of LILRA5.