LIMK1 Polyclonal antibody

LIMK1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 19699-1-AP


LIM domain kinase 1, LIMK, LIMK 1, LIMK1

Tested Applications

Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

19699-1-AP targets LIMK1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name LIM domain kinase 1
Calculated Molecular Weight 73 kDa
GenBank Accession NumberNM_002314
Gene Symbol LIMK1
Gene ID (NCBI) 3984
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.


Product Specific Protocols
IHC protocol for LIMK1 antibody 19699-1-APDownload protocol
IF protocol for LIMK1 antibody 19699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Dis

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Authors - Yue Zhang

Commun Biol

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Authors - Haixia Zhu

Oncol Rep

FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression.

Authors - Xiufang Yang

Cancer Med

MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Authors - Nengquan Sheng

Cell Death Discov

FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.

Authors - Xinyan Jiao

Mol Carcinog

LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling

Authors - Guanghui Hu