LIMK1 Polyclonal antibody

LIMK1 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19699-1-AP

Synonyms

LIM domain kinase 1, LIMK, LIMK 1, LIMK1



Tested Applications

Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19699-1-AP targets LIMK1 in IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name LIM domain kinase 1
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa, 33 kDa
GenBank accession numberNM_002314
Gene symbol LIMK1
Gene ID (NCBI) 3984
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

Protocols

Product Specific Protocols
IHC protocol for LIMK1 antibody 19699-1-APDownload protocol
IF protocol for LIMK1 antibody 19699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Authors - Nengquan Sheng
humanIHC

Oncol Rep

FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression.

Authors - Xiufang Yang
humanWB

Cell Death Dis

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Authors - Yue Zhang