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- KD/KO Validated
LIMPII Polyclonal antibody
LIMPII Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27102-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells, HepG2 cells, mouse liver tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 2 publications below |
Product Information
The immunogen of 27102-1-AP is LIMPII Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LIMPII fusion protein Ag25908 |
Full Name | scavenger receptor class B, member 2 |
Calculated molecular weight | 478 aa, 54 kDa |
Observed molecular weight | 75-85 kDa |
GenBank accession number | BC021892 |
Gene symbol | SCARB2 |
Gene ID (NCBI) | 950 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for LIMPII antibody 27102-1-AP | Download protocol |
IHC protocol for LIMPII antibody 27102-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Virus Res Murine models of neonatal susceptibility to a clinical strain of enterovirus A71 | ||
Viruses A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice.
| ||
Infect Genet Evol Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.
| ||
J Neurovirol Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells. | ||
PLoS Pathog The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness |