LIMPII Polyclonal antibody

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  WB analysis using 27102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 27102-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27102-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27102-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27102-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

LIMPII Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27102-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, NIH/3T3 cells, Y79 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

27102-1-AP targets LIMPII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIMPII fusion protein Ag25908
• Full Name: scavenger receptor class B, member 2
• Calculated Molecular Weight: 478 aa, 54 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC021892
• Gene Symbol: SCARB2
• Gene ID (NCBI): 950
• RRID: AB_2880756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14108
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Virus Res; Murine models of neonatal susceptibility to a clinical strain of enterovirus A71; Authors - Jing Xie
human	WB	J Virol; Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.; Authors - Zhichao Pei
mouse	WB,IHC	Viruses; A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice.; Authors - Xianliang Ke ; KO Validated
mouse	WB, IHC	Infect Genet Evol; Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.; Authors - Qingyuan Feng ; KD Validated
mouse	WB	J Neurovirol; Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells.; Authors - Wenjing Wang
human	WB	PLoS Pathog; The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness; Authors - Elena Sugrue