LIMPII Polyclonal antibody

• Featured Product
• KD/KO Validated

LIMPII Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27102-1-AP

Synonyms

AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 27102-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 27102-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27102-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27102-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27102-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HepG2 cells, mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 27102-1-AP is LIMPII Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIMPII fusion protein Ag25908
• Full Name: scavenger receptor class B, member 2
• Calculated molecular weight: 478 aa, 54 kDa
• Observed molecular weight: 75-85 kDa
• GenBank accession number: BC021892
• Gene symbol: SCARB2
• Gene ID (NCBI): 950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Virus Res; Murine models of neonatal susceptibility to a clinical strain of enterovirus A71; Authors - Jing Xie
human	WB	J Virol; Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.; Authors - Zhichao Pei
mouse	WB,IHC	Viruses; A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice.; Authors - Xianliang Ke ; KO Validated
mouse	WB, IHC	Infect Genet Evol; Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.; Authors - Qingyuan Feng ; KD Validated
mouse	WB	J Neurovirol; Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells.; Authors - Wenjing Wang
human	WB	PLoS Pathog; The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness; Authors - Elena Sugrue