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  • KD/KO Validated

LIMPII Polyclonal antibody

LIMPII Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27102-1-AP

Synonyms

AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, NIH/3T3 cells, Y79 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

27102-1-AP targets LIMPII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIMPII fusion protein Ag25908
Full Name scavenger receptor class B, member 2
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC021892
Gene symbol SCARB2
Gene ID (NCBI) 950
RRIDAB_2880756
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LIMPII antibody 27102-1-APDownload protocol
IHC protocol for LIMPII antibody 27102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Virus Res

Murine models of neonatal susceptibility to a clinical strain of enterovirus A71

Authors - Jing Xie
humanWB

J Virol

Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.

Authors - Zhichao Pei
mouseWB,IHC

Viruses

A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice.

Authors - Xianliang Ke
  • KO Validated
mouseWB, IHC

Infect Genet Evol

Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.

Authors - Qingyuan Feng
  • KD Validated
mouseWB

J Neurovirol

Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells.

Authors - Wenjing Wang
humanWB

PLoS Pathog

The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness

Authors - Elena Sugrue