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LIMS1 Polyclonal antibody

LIMS1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Quantity

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What's the best dilution for LIMS1 in ?

Tested Applications

Positive WB detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55336-1-AP targets LIMS1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name LIM and senescent cell antigen-like domains 1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberNM_004987
Gene Symbol LIMS1
Gene ID (NCBI) 3987
RRIDAB_2881311
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48059
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LIMS1 antibody 55336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The LIMS1 Polyclonal antibody (Cat# 55336-1-AP) has 1 citation. It appears in Journal of Cell Science (2018), in a study investigating how Kindlin-2 interacts with ILK to regulate focal adhesion localization and cell spreading. The research field is cell adhesion and cytoskeletal biology, with the antibody used for Western blotting in human samples.

SpeciesApplicationTitle
humanWB

J Cell Sci

Kindlin-2 interacts with a highly-conserved surface of ILK to regulate focal adhesion localization and cell spreading.

Authors - Yasmin A Kadry
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