LIN9 Polyclonal antibody

LIN9 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17882-1-AP

Synonyms

BARA, BARPsv, hLin 9, HuLin 9, Lin 9, lin 9 homolog (C. elegans), LIN9, pRB associated protein, Protein lin 9 homolog, TGS, TGS1, TUDOR gene similar protein

---

Validation Data Gallery

• 

  WB analysis using 17882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17882-1-AP (LIN9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 17882-1-AP

  IP result of anti-LIN9 (IP:17882-1-AP, 4ug; Detection:17882-1-AP 1:1000) with HEK-293 cells lysate 1100ug.

---

Tested Applications

• Positive WB detected in: MDA-MB-231 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• CoIP: See 1 publications below

Product Information

17882-1-AP targets LIN9 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIN9 fusion protein Ag11889
• Full Name: lin-9 homolog (C. elegans)
• Calculated Molecular Weight: 558 aa, 64 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC045625
• Gene Symbol: LIN9
• Gene ID (NCBI): 286826
• RRID: AB_10859819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN9, also named as BARA or TGS, is a 542 amino acid protein, which belongs to the lin9 family. LIN9 localizes in the nucleus and is expressed in thymus and testis. LIN9 acts as a tumor suppressor and inhibits DNA synthesis. LIN9 plays a role in the expression of genes, which required for the G1/S transition.

Publications

Species	Application	Title
		Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen
human	WB	Sci China Life Sci; PMID: 31420851; Authors - Hongna Lai
human	WB	EMBO Mol Med; Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer; Authors - Laura Pascual-Reguant
mouse	WB	FEBS Open Bio; The dual role of the DREAM/G2M pathway in non-tumorigenic immortalization of senescent cells; Authors - Jie Tian
human	WB	J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan ; KD Validated
mouse	WB,CoIP	Cell Death Discov; p21 Regulates Wnt-Notch balance via DREAM/MMB/Rb-E2F1 and maintains intestinal stem cell homeostasis; Authors - Liangxia Jiang