LIPM Polyclonal antibody

LIPM Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18817-1-AP

Synonyms

Lipase member M, lipase, family member M, LIPL3, LIPM

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Validation Data Gallery

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  IHC staining of human skin using 18817-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18817-1-AP (LIPM Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18817-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18817-1-AP (LIPM Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18817-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18817-1-AP (LIPM antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse skin using 18817-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18817-1-AP (LIPM antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18817-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18817-1-AP (LIPM antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 18817-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18817-1-AP (LIPM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 18817-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18817-1-AP (LIPM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human skin tissue, mouse skin tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18817-1-AP targets LIPM in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lipase, family member M
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC157888
• Gene Symbol: LIPM
• Gene ID (NCBI): 340654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIPM, also named as LIPL3, belongs to the AB hydrolase superfamily and Lipase family. LIPM plays a highly specific role in the last step of keratinocyte differentiation. LIPM may have an essential function in lipid metabolism of the most differentiated epidermal layers.