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Lamin B1 Antibody 35 Publications

Mouse Monoclonal| Catalog number: 66095-1-Ig |CloneNo.: 3C10G12

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Con: 161 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

Lamin B1 Antibody WB HepG2 cells 66095-1-Ig

WB result of Lamin B1 antibody (66095-1-Ig, 1:300,000) with si-Control and si-Lamin B1 transfected HepG2 cells.

Lamin B1 Antibody WB multi-cells/tissue 66095-1-Ig

Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000.

Lamin B1 Antibody IF HepG2 cells 66095-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 66095-1-Ig(Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Lamin B1 Antibody IHC human pancreas cancer tissue 66095-1-Ig

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig( Lamin B1 antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Lamin B1 Antibody FC HeLa cells 66095-1-Ig

1X10^6 HeLa cells were stained with 0.2ug Lamin B1 antibody (66095-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Lamin B1 Antibody WB HeLa cells 66095-1-Ig

Western blot of Hela cell with anti-LMNB1 (66095-1-Ig) at various dilutions.

Lamin B1 Antibody IHC human pancreas cancer tissue 66095-1-Ig

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig( Lamin B1 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Lamin B1 Antibody IP HeLa cells 66095-1-Ig

IP Result of anti-Lamin B1 (IP:66095-1-Ig, 4ug; Detection:66095-1-Ig 1:20000) with HeLa cells lysate 3560ug.

Species specificity:
human, mouse, rat, Rabbit
Positive WB detected in:
HeLa cells, multi-cells/tissue , HepG2 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human pancreas cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, HeLa cells
Positive FC detected in:
HeLa cells
ApplicationNote:
I recently tried the mouse (antibody 66095-1-Ig) and it works great. (by Stellrecht, Christine)
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:500-1:2000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Lamin B1 fusion protein Ag20522
Full name:
lamin B1
Calculated molecular weight:
66 kDa
Observed molecular weight:
66-70 kDa
GenBank accession number:
BC012295
Gene ID (NCBI):
4001
Gene symbol
LMNB1
Synonyms
ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

Background

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.


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