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Lamin B1 Monoclonal antibody
Lamin B1 Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
114
CloneNo.
3C10G12
Cat no : 66095-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | multi-cells/tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:250-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 100 publications below |
| IF | See 7 publications below |
Product Information
The immunogen of 66095-1-Ig is Lamin B1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, Rabbit, rat, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Lamin B1 fusion protein Ag20522 |
| Full Name | lamin B1 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66-70 kDa |
| GenBank accession number | BC012295 |
| Gene symbol | LMNB1 |
| Gene ID (NCBI) | 4001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| IF protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| IP protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| FC protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Rosmarinic acid protects mice from lipopolysaccharide/d-galactosamine-induced acute liver injury by inhibiting MAPKs/NF-κB and activating Nrf2/HO-1 signaling pathways. | ||
J Cell Physiol Fibrinopeptide A induces C-reactive protein expression through the ROS-ERK1/2/p38-NF-κB signal pathway in the human umbilical vascular endothelial cells. | ||
Nitric Oxide S-1-Propenylcysteine augments BACH1 degradation and heme oxygenase 1 expression in a nitric oxide-dependent manner in endothelial cells. | ||
J Proteome Res Multi-omic Analyses Reveal Minimal Impact of the CRISPR-Cas9 Nuclease on Cultured Human Cells. | ||
Nat Commun The meiotic TERB1-TERB2-MAJIN complex tethers telomeres to the nuclear envelope. | ||
Mol Cell Biol Constitutive p38 activation caused inactivation of CRE transcription is mediated by hyper-phosphorylation dependent CRTC2 nucleocytoplasmic transport. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Eiko (Verified Customer) (11-18-2020) | Used RIPA buffer for western sample preparation, and 5% Skim milk TBS-T was used for blocking and antibody dilution.Since I could see clear lamin B1 signal, I am happy to use this antibody as one of my internal controls.
|
FH Tom (Verified Customer) (10-09-2020) | I observed a discrete ~70kDa Lamin B1 band using this antibody.
|
FH Yuan (Verified Customer) (12-13-2019) | Did staining for human A549 cell.The Lamin B antibody had high background in nucleus. Please see attached image.
 |
FH Shubham (Verified Customer) (03-14-2019) | good
 |
