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CoraLite®647-conjugated Lamin B1 Monoclonal antibody

Lamin B1 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

3C10G12

Cat no : CL647-66095

Synonyms

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66095-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL647-66095 is Lamin B1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Lamin B1 fusion protein Ag20522
Full Name lamin B1
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC012295
Gene symbol LMNB1
Gene ID (NCBI) 4001
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Product Specific Protocols
IF protocol for CL647 Lamin B1 antibody CL647-66095Download protocol
FC protocol for CL647 Lamin B1 antibody CL647-66095Download protocol
Standard Protocols
Click here to view our Standard Protocols