|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of CL594-66095 is Lamin B1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Lamin B1 fusion protein Ag20522|
|Full Name||lamin B1|
|Calculated molecular weight||66 kDa|
|GenBank accession number||BC012295|
|Gene ID (NCBI)||4001|
|Conjugate||CoraLite®594 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||593 nm / 614 nm|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
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