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CoraLite®555-conjugated Lamin B1 Monoclonal antibody
Lamin B1 Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
3C10G12
Cat no : CL555-66095
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | MCF-7 cells, U2OS cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL555-66095 is Lamin B1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Lamin B1 fusion protein Ag20522 |
Full Name | lamin B1 |
Calculated molecular weight | 66 kDa |
Observed molecular weight | 66-70 kDa |
GenBank accession number | BC012295 |
Gene symbol | LMNB1 |
Gene ID (NCBI) | 4001 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.
Protocols
Product Specific Protocols | |
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IF protocol for CL555 Lamin B1 antibody CL555-66095 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |