Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, Raji cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells, SH-SY5Y cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10895-1-AP targets Lamin B2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1335 Product name: Recombinant human LMNB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-600 aa of BC006551 Sequence: EELRSQHDEQVRLYKLELEQTYQAKLDSAKLSSDQNDKAASAAREELKEARMRLESLSYQLSGLQKQASAAEDRIRELEEAMAGERDKFRKMLDAKEQEMTEMRDVMQQQLAEYQELLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSGSLSATGRLGRSKRKRLEVEEPLGSGPSVLGTGTGGSGGFHLAQQASASGSVSIEEIDLEGKFVQLKNNSDKDQSLGNWRIKRQVLEGEEIAYKFTPKYILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM Predict reactive species |
| Full Name | lamin B2 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 66-68 kDa |
| GenBank Accession Number | BC006551 |
| Gene Symbol | Lamin B2 |
| Gene ID (NCBI) | 84823 |
| RRID | AB_2136411 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| IHC protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| IP protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| WB protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Lamin B2 Polyclonal antibody (Cat# 10895-1-AP) has 37 citations. These appear across journals including Nature Cell Biology, Molecular Cell, Nature Chemical Biology, Science Advances, Pharmacological Research, Phytomedicine, Cell Death & Discovery, Environmental International, PNAS, Oncogenesis, Cell Reports, and Experimental Cell Research, among others. Research fields span nuclear biology, cancer (hepatocellular, melanoma, colorectal, glioma), DNA repair, apoptosis, oxidative stress, inflammation, and cell cycle regulation. Applications are predominantly Western blot, with some IF, IHC, and Co-IP.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion
| ||
Mol Cell MLKL activates the cGAS-STING pathway by releasing mitochondrial DNA upon necroptosis induction | ||
Nat Chem Biol Fructose-1,6-bisphosphatase 1 dephosphorylates and inhibits TERT for tumor suppression | ||
Sci Adv 53BP1 orchestrates sequence feature of RAG targets to balance DNA repair outcomes during V(D)J recombination. | ||
Pharmacol Res YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the Mediator complex in colorectal cancer | ||
Phytomedicine Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts |
Reviews
What customers say
The single available review reports using this antibody for Western Blot at a 1:1000 dilution. The reviewer observed specific bands at the predicted molecular weight, though some weak non-specific bands were also present. Overall, the product was rated 4 out of 5 stars, indicating generally satisfactory performance with minor background concerns.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
WEI (Verified Customer) (03-08-2022) | Specific bands at predicted size with weak non-specific bands.
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