Validation Data Gallery
|Positive WB detected in||HepG2 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, SH-SY5Y cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10895-1-AP targets Lamin B2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Lamin B2 fusion protein Ag1335|
|Full Name||lamin B2|
|Calculated molecular weight||68 kDa|
|Observed molecular weight||66-68 kDa|
|GenBank accession number||BC006551|
|Gene ID (NCBI)||84823|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for Lamin B2 antibody 10895-1-AP||Download protocol|
|IHC protocol for Lamin B2 antibody 10895-1-AP||Download protocol|
|IF protocol for Lamin B2 antibody 10895-1-AP||Download protocol|
|IP protocol for Lamin B2 antibody 10895-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (03-08-2022)
Specific bands at predicted size with weak non-specific bands.