LMO4 Polyclonal antibody

LMO4 Polyclonal Antibody for IHC, IP, ELISA

Cat No. 12146-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA

Synonyms

LMO 4, LMO-4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Filter: AllIPIHC

IP experiment of T-47D using 12146-1-AP
IP result of anti-LMO4 (IP:12146-1-AP, 4ug; Detection:12146-1-AP 1:500) with T-47D cells lysate 1120 ug.
IHC staining of mouse brain using 12146-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12146-1-AP (LMO4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Positive IP detected in	T-47D cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

12146-1-AP targets LMO4 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2794 Product name: Recombinant human LMO4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-165 aa of BC017673 Sequence: MVNPGSSSQPPPVTAGSLSWKRCAGCGGKIADRFLLYAMDSYWHSRCLKCSCCQAQLGDIGTSCYTKSGMILCRNDYIRLFGNSGACSACGQSIPASELVMRAQGNVYHLKCFTCSTCRNRLVPGDRFHYINGSLFCEHDRPTALINGHLNSLQSNPLLPDQKVC Predict reactive species
Full Name	LIM domain only 4
Calculated Molecular Weight	165 aa, 18 kDa
GenBank Accession Number	BC017673
Gene Symbol	LMO4
Gene ID (NCBI)	8543
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P61968
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.