LNPEP Polyclonal antibody

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  WB analysis of human brain using 55154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 55154-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55154-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 40x lens).

LNPEP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55154-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CAP, Cystinyl aminopeptidase, IRAP, Leucyl cystinyl aminopeptidase, leucyl/cystinyl aminopeptidase, LNPEP, OTase, Oxytocinase, P LAP, PLAP, VP165

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Tested Applications

• Positive WB detected in: human brain tissue, COLO 320 cells, human heart tissue
• Positive IHC detected in: human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55154-1-AP targets LNPEP in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: leucyl/cystinyl aminopeptidase
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 117-125 kDa
• GenBank Accession Number: NM_005575
• Gene Symbol: LNPEP
• Gene ID (NCBI): 4012
• RRID: AB_10860904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.