LNX1 Polyclonal antibody

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  WB analysis of mouse testis using 13124-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13124-1-AP (LNX1 antibody) at dilution of 1:70 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13124-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13124-1-AP (LNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

LNX1 Polyclonal Antibody for WB, ELISA

Cat No. 13124-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ligand of numb protein X 1, LNX, LNX1, MPDZ, Numb binding protein 1, PDZRN2

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13124-1-AP targets LNX1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3823

  Product name: Recombinant human LNX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 378-728 aa of BC022983

  Sequence: DSFHVILNKSSPEEQLGIKLVRKVDEPGVFIFNVLDGGVAYRHGQLEENDRVLAINGHDLRYGSPESAAHLIQASERRVHLVVSRQVRQRSPDIFQEAGWNSNGSWSPGPGERSNTPKPLHPTITCHEKVVNIQKDPGESLGMTVAGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVDGVELTEVSRSEAVALLKRTSSSIVLKALEVKEYEPQEDCSSPAALDSNHNMAPPSDWSPSWVMWLELPRCLYNCKDIVLRRNTAGSLGFCIVGGYEEYNGNKPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARLLKELKGRITLTIVSWPGTFL

• Full Name: ligand of numb-protein X 1
• Calculated Molecular Weight: 728 aa, 81 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC022983
• Gene Symbol: LNX1
• Gene ID (NCBI): 84708
• RRID: AB_2136987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TBB1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LNX1 (Ligand of Numb, protein X 1) was originally identified as a binding partner of the cell-fate determinant Numb and was reported to be involved in ubiquitylation and degradation of Numb. LNX1 contains four PDZ domains and may function as a scaffold for a multi-protein complex. LNX1 has two splicing variants, LNX1p70 and LNX1p80. LNX1p70 has the N-terminal Numb binding region and four PDZ domains, whereas LNX1p80 has an additional RING-finger domain at the N-terminus followed by a Numb-binding region and four PDZ domains. This antibody can detect both LNX1p70 and LNX1p80, around 70-80 kDa and 80-100 kDa, respectively.