Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18035-1-AP targets LONP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12650 Product name: Recombinant human LONP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 503-852 aa of BC093910 Sequence: DRMEIIQVPGYTQEEKIEIAHRHLIPKQLEQHGLTPQQIQIPQVTTLDIITRYTREAGVRSLDRKLGAICRAVAVKVAEGQHKEAKLDRSDVTEREGCREHILEDEKPESISDTTDLALPPEMPILIDFHALKDILGPPMYEMEVSQRLSQPGVAIGLAWTPLGGEIMFVEASRMDGEGQLTLTGQLGDVMKESAHLAISWLRSNAKKYQLTNAFGSFDLLDNTDIHLHFPAGAVTKDGPSAGVTIVTCLASLFSGRLVRSDVAMTGEITLRGLVLPVGGIKDKVLAAHRAGLKQVIIPRRNEKDLEGIPGNVRQDLSFVTASCLDEVLNAAFDGGFTVKTRPGLLNSKL Predict reactive species |
| Full Name | lon peptidase 2, peroxisomal |
| Calculated Molecular Weight | 852 aa, 95 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC093910 |
| Gene Symbol | LONP2 |
| Gene ID (NCBI) | 83752 |
| RRID | AB_2878485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LONP2 (Lon Peptidase 2, Peroxisomal) is an important ATP-dependent protease. It is primarily located in peroxisomes and is responsible for maintaining the proteostasis of this organelle. By degrading misfolded proteins and selectively regulating metabolic enzymes, LONP2 plays a central role in maintaining peroxisomal proteostasis and metabolic balance. Dysfunction of LONP2 is closely related to a variety of human diseases, including peroxisomal disorders, cancer, and neurodegenerative diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LONP2 antibody 18035-1-AP | Download protocol |
| IHC protocol for LONP2 antibody 18035-1-AP | Download protocol |
| WB protocol for LONP2 antibody 18035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-LONP2 antibody (Cat# 18035-1-AP) has 2 citations in total. These appear in the journals Gene and Cancer Management Research. The associated research fields include head and neck squamous cell carcinoma and cervical cancer, with one study investigating LONP2's role in enhancing cervical cancer growth and metastatic potential via PLS3 upregulation and PI3K/AKT signaling activation. Applications used were WB and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Cancer Manag Res LONP2 Enhances Cervical Cancer Growth and Metastatic Potential by Upregulating PLS3 and Activating PI3K/AKT Signaling. |
