Loricrin Polyclonal antibody

Loricrin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 55439-1-AP

Synonyms

LOR, LRN

---

Validation Data Gallery

• 

  WB analysis of A431 using 55439-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55439-1-AP (Loricrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human paracancerous using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded paracancerous slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human paracancerous tissue of skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of skin slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human paracancerous tissue of skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of skin slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 55439-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Loricrin antibody (55439-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: rat skin tissue, human paracancerous, human paracancerous tissue of skin, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 10 publications below

Product Information

55439-1-AP targets Loricrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: loricrin
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM_000427
• Gene Symbol: Loricrin
• Gene ID (NCBI): 4014
• RRID: AB_2881332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.

Publications

Species	Application	Title
mouse	WB,IF	Cell Death Differ; ENKD1 promotes epidermal stratification by regulating spindle orientation in basal keratinocytes.; Authors - Tao Zhong
mouse	WB	EMBO Rep; Regulation of epidermal differentiation through KDF1-mediated deubiquitination of IKKα.; Authors - Yuanyuan Li
human,mouse	WB,IF	Br J Pharmacol; FGF21 promotes migration and differentiation of epidermal cells during wound healing via SIRT1 dependent autophagy.; Authors - Xixi Chen
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
mouse	WB,IF	J Ethnopharmacol; Topical application of Artemisia annua L. essential oil ameliorates 2,4-dintrochlorobenzene-induced atopic dermatitis in mice; Authors - Zhiya Huang
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan

Related Products

TGM1 Polyclonal antibody

12912-3-AP

VEGFR2/KDR Polyclonal antibody

26415-1-AP

GJB3 Polyclonal antibody

12880-1-AP

SPRR3 Polyclonal antibody

11742-1-AP

Cytokeratin 1-specific Polyclonal antibody

16848-1-AP

Involucrin Polyclonal antibody

55328-1-AP

Envoplakin Polyclonal antibody

25441-1-AP