LOXL2 Monoclonal antibody
LOXL2 Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1H7F1
Cat no : 67139-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, human placenta tissue, A375 cells, human heart tissue |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IF | See 1 publications below |
Product Information
67139-1-Ig targets LOXL2 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | LOXL2 fusion protein Ag28566 |
Full Name | lysyl oxidase-like 2 |
Calculated molecular weight | 774 aa, 87 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC000594 |
Gene symbol | LOXL2 |
Gene ID (NCBI) | 4017 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
IF protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging Cell Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis. | ||
Int J Biol Macromol Depletion of LOXL2 improves respiratory capacity: From air-breathing fish to mammal under hypoxia. | ||
Oncogene METTL1/WDR4-mediated tRNA m7G modification and mRNA translation control promote oncogenesis and doxorubicin resistance |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (10-04-2021) | The calculated MW is 87 kd. This antibody recognized major bands at 65, and 60 kd.
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