EDG7-Specific Polyclonal antibody

EDG7-Specific Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 19509-1-AP

Synonyms

Edg 7, EDG7, GPCR, HOFNH30, LP A3, LPA 3, LPA receptor 3, LPA3, LPAR3, RP4 678I3



Tested Applications

Positive WB detected inPC-3 cells, human testis tissue, mouse pancreas tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

19509-1-AP targets EDG7-Specific in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lysophosphatidic acid receptor 3
Calculated molecular weight 40 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberNM_012152
Gene symbol LPAR3
Gene ID (NCBI) 23566
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LPAR3, also named as EDG7 and LPA3, belongs to the G-protein coupled receptor 1 family. LPAR3 is a receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. LPAR3 may play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins. The antibody is specific to LPAR3.

Protocols

Product Specific Protocols
WB protocol for EDG7-Specific antibody 19509-1-APDownload protocol
IHC protocol for EDG7-Specific antibody 19509-1-APDownload protocol
FC protocol for EDG7-Specific antibody 19509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Comput Struct Biotechnol J

Interrelations of Sphingolipid and Lysophosphatidate Signaling with Immune System in Ovarian Cancer.

Authors - Anastasia Meshcheryakova