EDG7-Specific Polyclonal antibody

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  WB analysis of PC-3 using 19509-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19509-1-AP (EDG7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 19509-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19509-1-AP (EDG7-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19509-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19509-1-AP (EDG7-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19509-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19509-1-AP (EDG7-Specific antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 19509-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19509-1-AP (EDG7-Specific antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse heart using 19509-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19509-1-AP (EDG7-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EDG7-Specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19509-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Edg 7, EDG7, GPCR, HOFNH30, LP A3, LPA 3, LPA receptor 3, LPA3, LPAR3, RP4 678I3

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Tested Applications

• Positive WB detected in: PC-3 cells, human testis tissue, mouse pancreas tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

19509-1-AP targets EDG7-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: lysophosphatidic acid receptor 3
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: NM_012152
• Gene Symbol: LPAR3
• Gene ID (NCBI): 23566
• RRID: AB_10641042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBY5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPAR3, also named as EDG7 and LPA3, belongs to the G-protein coupled receptor 1 family. LPAR3 is a receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. LPAR3 may play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins. The antibody is specific to LPAR3.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Comput Struct Biotechnol J; Interrelations of Sphingolipid and Lysophosphatidate Signaling with Immune System in Ovarian Cancer.; Authors - Anastasia Meshcheryakova
mouse	WB	Pediatr Res; Bronchopulmonary dysplasia demonstrates dysregulated autotaxin/lysophosphatidic acid signaling in a neonatal mouse model; Authors - Alison W Ha
human	IF	Nat Commun; Time-series single-cell transcriptomic profiling of luteal-phase endometrium uncovers dynamic characteristics and its dysregulation in recurrent implantation failures; Authors - Dandan Cao