Filter: AllIHCIF-P

IHC staining of mouse brain using 30142-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 30142-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 30142-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 30142-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 30142-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 30142-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 30142-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LPHN1 Polyclonal antibody

Name: LPHN1 Polyclonal antibody
Brand: Proteintech
SKU: 30142-1-AP

LPHN1 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 30142-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

CIRL-1, CIRL1, CIRL 1, Calcium-independent alpha-latrotoxin receptor 1, Adhesion G protein-coupled receptor L1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in	mouse brain tissue, rat kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30142-1-AP targets LPHN1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag31093 Product name: Recombinant human LPHN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 400-500 aa of NM_001008701 Sequence: DPSAGPATSPPLSTTTTARPTPLTSTASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPVPSTRRPPAPNLHVSPELFCEPREVRRVQWPATQQGMLVE Predict reactive species
Full Name	latrophilin 1
Calculated Molecular Weight	163 kDa
GenBank Accession Number	NM_001008701
Gene Symbol	LPHN1
Gene ID (NCBI)	22859
RRID	AB_3742328
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94910
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Latrophilin-1 (LPHN1) was isolated as the main high-affinity receptor for α-latrotoxin from black widow spider venom, a powerful presynaptic secretagogue (PMID: 30967757). aGPCR latrophilin 1 (ADGRL1/LPHN1) is involved in controlling energy balance and food intake. Although this receptor is present in adipocytes and many neurons, where it is essential for axon growth cone formation and neuronal. It is present in Adipocytes and many neurons, where it is essential for Axon growth cone formation and neuronal function (PMID: 38664368).