LPHN2 Polyclonal antibody

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  IHC staining of mouse cerebellum using 20043-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20043-1-AP (LPHN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20043-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20043-1-AP (LPHN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 20043-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using LPHN2 antibody (20043-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

LPHN2 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 20043-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

CIRL-2, CIRL2, CIRL 2, Calcium-independent alpha-latrotoxin receptor 2, Adhesion G protein-coupled receptor L2

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Tested Applications

• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20043-1-AP targets LPHN2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: latrophilin 2
• Calculated Molecular Weight: 163 kDa
• GenBank Accession Number: NM_012302
• Gene Symbol: LPHN2
• Gene ID (NCBI): 23266
• RRID: AB_3085596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95490
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPHN2, Latrophilin-2, is a calcium-independent receptor of low affinity for alpha-latrotoxin which triggers massive exocytosis from neurons and neuroendocrine cells. LPHN2 is a novel receptor of LRG1 that rescues vascular and neurological abnormalities (PMID: 35562586). LPHN2 and LPHN3 serve as postsynaptic recognition molecules in CA1 pyramidal neurons of the hippocampus, localized to Purkinje cells in cerebellum (PMID: 32202499).