LPHN3 Polyclonal antibody

LPHN3 Polyclonal Antibody for WB, IP, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20045-1-AP

Synonyms

CIRL 3, CIRL3, KIAA0768, latrophilin 3, LEC3, Lectomedin 3, LPHN3

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Validation Data Gallery

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  WB analysis of mouse brain using 20045-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20045-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20045-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20045-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 20045-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20045-1-AP

  IP result of anti-LPHN3 (IP:20045-1-AP, 4ug; Detection:20045-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  FC experiment of HepG2 using 20045-1-AP

  1X10^6 HepG2 cells were stained with .2ug LPHN3 antibody (20045-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, mouse colon tissue, COLO 320 cells, rat brain tissue, human liver tissue
• Positive IP detected in: mouse brain tissue
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20045-1-AP targets LPHN3 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: latrophilin 3
• Calculated Molecular Weight: 162 kDa
• Observed Molecular Weight: 139 kDa
• GenBank Accession Number: NM_015236
• Gene Symbol: LPHN3
• Gene ID (NCBI): 23284
• RRID: AB_10665552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.

Publications

Species	Application	Title
human	WB	World J Biol Psychiatry; Expression of the adult ADHD-associated gene ADGRL3 is dysregulated by risk variants and environmental risk factors.; Authors - Rhiannon V McNeill