LPO Polyclonal antibody

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• LPO Polyclonal antibody (10376-1-AP)

LPO Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10376-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

lactoperoxidase, LPO, Salivary peroxidase, SAPX, SPO

• 

  WB analysis of human saliva using 10376-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 10376-1-AP (LPO Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 10376-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10376-1-AP (LPO Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 10376-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human saliva tissue, K-562 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10376-1-AP targets LPO in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  peptide

• Full Name: lactoperoxidase
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC107167
• Gene Symbol: LPO
• Gene ID (NCBI): 4025
• RRID: AB_2877734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22079
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Anti-TPO (Thyroid Peroxidase) antibody, catalog number 10376-1-AP, has 8 total citations. These appear in Nature Aging, Cell Death Discovery, Diabetologia, Frontiers in Cell and Developmental Biology, FASEB Journal, Free Radical Research, and PLoS One. Research fields span thymus function and mTOR signaling, podocyte ferroptosis, diabetes and metformin therapy, colon cancer prognosis, atherosclerosis, fatty liver disease, and thyroid/breast tissue TPO characterization.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Aging; Paracrine FGF21 dynamically modulates mTOR signaling to regulate thymus function across the lifespan; Authors - Sarah A Wedemeyer
mouse	WB	Cell Death Discov; MAPK14/SLC7A11/GPX4 axis dysregulation drives podocyte ferroptosis via mediating glycerophospholipid metabolism.; Authors - Shi Qiu
		Diabetologia; Metformin protects podocytes by inhibiting the MAPK14-SLC7A11-GPX4 axis and dysregulation of fatty acid metabolism.; Authors - Shi Qiu
human	IHC	Front Cell Dev Biol; Identification and verification of a prognostic signature based on a miRNA-mRNA interaction pattern in colon adenocarcinoma; Authors - Qiwu Zhao
mouse	WB	FASEB J; Melatonin suppresses atherosclerosis by ferroptosis inhibition via activating NRF2 pathway; Authors - Yangyang Tao
human	WB	Free Radic Res; Metformin suppresses metabolic dysfunction-associated fatty liver disease by ferroptosis and apoptosis via activation of oxidative stress; Authors - Zhiyu Li