Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 25045-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 25045-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 25045-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 25045-1-AP
IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.
IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.
IHC staining of human small intestine using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon, mouse heart, rat heart
Positive IP detected in
HeLa cells
Positive IHC detected in
human small intestine tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 25045-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 25045-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 25045-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human small intestine using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of HeLa using 25045-1-AP
IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.
IF/ICC Figures
IF Staining of HeLa using 25045-1-AP
Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
LPP Polyclonal antibody
Catalog Number
25045-1-AP
Citations
1
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
