LPXN Polyclonal antibody

LPXN Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11307-1-AP

Synonyms

LDLP, LDPL, leupaxin, LPXN

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Validation Data Gallery

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  WB analysis of PC-3 using 11307-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11307-1-AP (LPXN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 11307-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11307-1-AP (LPXN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11307-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11307-1-AP (LPXN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11307-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11307-1-AP (LPXN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11307-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11307-1-AP (LPXN Antibody) at dilution of 1:50. (under 10x lens).

• 

  IHC staining of human prostate cancer using 11307-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11307-1-AP (LPXN Antibody) at dilution of 1:50. (under 40x lens).

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  IHC staining of human tonsillitis using 11307-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 11307-1-AP (LPXN Antibody) at dilution of 1:50. (under 10x lens).

• 

  IHC staining of human tonsillitis using 11307-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 11307-1-AP (LPXN Antibody) at dilution of 1:50. (under 40x lens).

• 

  IF Staining of PC-3 using 11307-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 11307-1-AP (LPXN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, RAW 264.7 cells
• Positive IHC detected in: human lung cancer tissue, human tonsillitis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

11307-1-AP targets LPXN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LPXN fusion protein Ag1845
• Full Name: leupaxin
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC019035
• Gene Symbol: LPXN
• Gene ID (NCBI): 9404
• RRID: AB_2877756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).

Publications

Species	Application	Title
mouse	IF	J Mol Cell Cardiol; Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.; Authors - Xinming Wang