Tested Applications
| Positive WB detected in | mouse testis tissue, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12815-1-AP targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3556 Product name: Recombinant human LRAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-201 aa of BC031053 Sequence: EDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVL Predict reactive species |
| Full Name | lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) |
| Calculated Molecular Weight | 230 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa, 50-54 kDa |
| GenBank Accession Number | BC031053 |
| Gene Symbol | LRAT |
| Gene ID (NCBI) | 9227 |
| RRID | AB_2297180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LRAT antibody 12815-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LRAT Polyclonal antibody (Cat# 12815-1-AP) has 9 citations published in journals including Phytomedicine, iScience, Am J Pathol, Biochim Biophys Acta Mol Basis Dis, J Cell Commun Signal, Toxicol Lett, Exp Eye Res, and J Food Biochem. Research fields span diabetic nephropathy, hepatic fibrosis, diabetic retinopathy, retinoic acid metabolism, vitamin A homeostasis, hepatic steatosis, retinal photoreceptor degeneration, and retinol bioavailability.
| Species | Application | Title |
|---|---|---|
Phytomedicine Integrated metabolomics and mass spectrometry imaging analysis reveal the efficacy and mechanism of Huangkui capsule on type 2 diabetic nephropathy | ||
Biochim Biophys Acta Mol Basis Dis The m6A reader IGF2BP2 promotes hepatic fibrosis by stabilizing AEBP1 mRNA and regulating GRB2-mediated hepatic stellate cell activation. | ||
J Cell Commun Signal Structural maintenance of chromosome protein 1A exacerbates liver fibrosis by enhancing hepatic stellate cell activation and extracellular matrix synthesis via laminin subunit gamma 2 activation. |
Reviews
What customers say
Both reviewers used this antibody for Western Blot with positive results. One user achieved good performance at a 1:1000 dilution in 1X TBST on mouse liver tissue (4/5). The other reported excellent results at 1:500 on rat liver tissue, noting that the BSA-based formulation allows the antibody to be reused multiple times when stored at -20°C, with consistent performance maintained over six months or more (5/5). Overall, users find it reliable and stable for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal (Verified Customer) (01-22-2026) | Antibody works when diluted 1:1000 in 1X TBST for western blot.
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Peter (Verified Customer) (12-03-2018) | The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.
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