LRAT Polyclonal antibody

LRAT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 12815-1-AP

Synonyms

LRAT

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12815-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12815-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12815-1-AP targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRAT fusion protein Ag3556
• Full Name: lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
• Calculated Molecular Weight: 230 aa, 26 kDa
• Observed Molecular Weight: 26 kDa, 50-54 kDa
• GenBank Accession Number: BC031053
• Gene Symbol: LRAT
• Gene ID (NCBI): 9227
• RRID: AB_2297180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

Publications

Species	Application	Title
rat	WB	iScience; Control by the brain of vitamin A homeostasis; Authors - Peter I Imoesi
rat	WB	Am J Pathol; Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.; Authors - Volha V Malechka
mouse	WB	J Food Biochem; Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.; Authors - Wei Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Peter (Verified Customer) (12-03-2018)

The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Liver Tissue

LRAT Antibody Western Blot, validation (1:500 dilution) in Rat Liver Tissue (Cat no:12815-1-AP)