Tested Applications
| Positive WB detected in | A431 cells, rat liver tissue, MCF-7 cells, SKOV-3 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human lung cancer tissue, human urothelial carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13079-1-AP targets PTPRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3760 Product name: Recombinant human LRP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 494-793 aa of BC027308 Sequence: LYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, A |
| Calculated Molecular Weight | 793 aa, 91 kDa |
| Observed Molecular Weight | 90-105 kDa, 140 kDa |
| GenBank Accession Number | BC027308 |
| Gene Symbol | PTPRA |
| Gene ID (NCBI) | 5786 |
| RRID | AB_2269055 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18433 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IHC protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IP protocol for PTPRA antibody 13079-1-AP | Download protocol |
| WB protocol for PTPRA antibody 13079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PTPRA (RPTPα) antibody (Cat# 13079-1-AP) has 4 total citations across four journals: EBioMedicine, Oncogene, Science Signaling, and Oncology Letters. Research fields span cancer biology (non-small cell lung cancer, breast cancer), N-glycosylation and Src signaling regulation, and rheumatoid arthritis pathogenesis in synovial fibroblasts. Applications include WB, IF, and IP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
EBioMedicine The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.
| ||
Oncogene Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation | ||
Sci Signal Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts | ||
Oncol Lett PTPRA facilitates cancer growth and migration via the TNF-α-mediated PTPRA-NF-κB pathway in MCF-7 breast cancer cells.
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