LRPPRC Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA sample test of MP02559-1

  The mean LRPPRC concentration was determined to be 181.06 ng/mL in HCT116 cell extract based on a 1.0 mg/mL extract load, 438.54 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 207.78 ng/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02559-1

  Sandwich ELISA standard curve of MP02559-1, Human LRPPRC Recombinant Matched Antibody Pair - PBS only. 86616-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15452. 86616-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Cytometric bead array sample test of MP02559-1

  The mean LRPPRC concentration was determined to be 67.2 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load, 168.4 ng/mL in HCT 116 cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP02559-1

  Cytometric bead array standard curve of MP02559-1, LRPPRC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86616-1-PBS. Detection antibody: 86616-2-PBS. Standard: Ag15452. Range: 1.562-100 ng/mL.

LRPPRC Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86616-1-PBS

Clone No.251577C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

130 kDa leucine-rich protein, GP130, Leucine-rich PPR motif-containing protein, mitochondrial, LRP 130, LRP130

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Product Information

86616-1-PBS targets LRPPRC as part of a matched antibody pair:

MP02559-1: 86616-1-PBS capture and 86616-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15452

  Product name: Recombinant human LRPPRC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1014-1394 aa of BC050311

  Sequence: LWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS

• Full Name: leucine-rich PPR-motif containing
• Calculated Molecular Weight: 1394 aa, 158 kDa
• GenBank Accession Number: BC050311
• Gene Symbol: LRPPRC
• Gene ID (NCBI): 10128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42704
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.