LRRC59 Polyclonal antibody

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• LRRC59 Polyclonal antibody (27208-1-AP)

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  WB analysis using 27208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27208-1-AP (LRRC59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27208-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27208-1-AP (LRRC59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27208-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27208-1-AP (LRRC59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LRRC59 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27208-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Leucine-rich repeat-containing protein 59, Leucine-rich repeat-containing protein 59, N-terminally processed, p34, PRO1855, Ribosome-binding protein p34

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

27208-1-AP targets LRRC59 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26090

  Product name: Recombinant human LRRC59 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-244 aa of BC052279

  Sequence: MTKAGSKGGNLRDKLDGNELDLSLSDLNEVPVKELAALPKATILDLSCNKLTTLPSDFCGLTHLVKLDLSKNKLQQLPADFGRLVNLQHLDLLNNKLVTLPVSFAQLKNLKWLDLKDNPLDPVLAKVAGDCLDEKQCKQCANKVLQHMKAVQADQERERQRRLEVEREAEKKREAKQRAKEAQERELRKREKAEEKERRRKEYDALKAAKREQEKKPKKEANQAPKSKSGSRPRKPPPRKHTRS

• Full Name: leucine rich repeat containing 59
• Calculated Molecular Weight: 307 aa, 35 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: BC052279
• Gene Symbol: LRRC59
• Gene ID (NCBI): 55379
• RRID: AB_2880801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AG4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Oncol Rep; LRRC59 inhibits perk pathway‑induced apoptosis and promotes cell proliferation, migration and invasion in colorectal cancer cells.; Authors - Xingdong Hou ; KD Validated
mouse	WB	Nat Commun; OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools; Authors - Ara Lee
human	WB	Aging (Albany NY); Pan-cancer analysis of LRRC59 with a focus on prognostic and immunological roles in hepatocellular carcinoma.; Authors - Boyu Pan ; KD Validated