LRRC8D Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HaCaT using 86510-1-RR (same clone as 86510-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 86510-1-RR (LRRC8D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86510-1-RR (same clone as 86510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86510-1-RR (LRRC8D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 86510-1-RR (same clone as 86510-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRRC8D antibody (86510-1-RR, Clone: 251257E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

LRRC8D Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86510-1-PBS

Clone No.251257E6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HsLRRC8D, Leucine-rich repeat-containing protein 5, Leucine-rich repeat-containing protein 8D, LRRC5, Volume-regulated anion channel subunit LRRC8D

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Product Information

86510-1-PBS targets LRRC8D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2138

  Product name: Recombinant human LRRC8D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 515-858 aa of BC024159

  Sequence: LQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQLFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPLEVKEALNQDINIPFANGI

• Full Name: leucine rich repeat containing 8 family, member D
• Calculated Molecular Weight: 858 aa, 98 kDa
• Observed Molecular Weight: 98-120 kDa
• GenBank Accession Number: BC024159
• Gene Symbol: LRRC8D
• Gene ID (NCBI): 55144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7L1W4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.