LRRTM3 Polyclonal antibody

LRRTM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27003-1-AP

Synonyms

LRRTM3

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Validation Data Gallery

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  WB analysis of Neuro-2a using 27003-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27003-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 27003-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 27003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 27003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27003-1-AP is LRRTM3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRTM3 fusion protein Ag25452
• Full Name: leucine rich repeat transmembrane neuronal 3
• Calculated Molecular Weight: 581 aa, 66 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC111492
• Gene Symbol: LRRTM3
• Gene ID (NCBI): 347731
• RRID: AB_2880719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.