LSP1 Polyclonal antibody

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  WB analysis of Raji using 15593-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 15593-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 15593-1-AP

  IP result of anti-LSP1 (IP:15593-1-AP, 4ug; Detection:15593-1-AP 1:300) with Raji cells lysate 4000ug.

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  IHC staining of human colon using 15593-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15593-1-AP (LSP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LSP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15593-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

47 kDa actin-binding protein, Lymphocyte-specific antigen WP34, Lymphocyte-specific protein 1, pp52, WP34

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Tested Applications

• Positive WB detected in: HL-60 cells, U-937 cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15593-1-AP targets LSP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7944

  Product name: Recombinant human LSP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-339 aa of BC001785

  Sequence: MAEASSDPGAEEREELLGPTAQWSVEDEEEAVHEQCQHERDRQLQAQDEEGGGHVPERPKQEMLLSLKPSEAPELDEDEGFGDWSQRPEQRQQHEGAQGTLDSGEPPQCRSPEGEQEDRPGLHAYEKEDSDEVHLEELSLSKEGPGPEDTVQDNLGAAGAEEEQEEHQKCQQPRTPSPLVLEGTIEQSSPPLSPTTKLIDRTESLNRSIEKSNSVKKSQPDLPISKIDQWLEQYTQAIETAGRTPKLARQASIELPSMAVASTKSRWETGEVQAQSAAKTPSCKDIVAGDMSKKSLWEQKGGSKTSSTIKSTPSGKRYKFVATGHGKYEKVLVEGGPAP

• Full Name: lymphocyte-specific protein 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001785
• Gene Symbol: LSP1
• Gene ID (NCBI): 4046
• RRID: AB_2250134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33241
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphocyte-specific protein 1(LSP1), also known as leukocyte-specific protein 1, is an F-actin binding and cytoskeleton associated protein. The protein is expressed in lymphocytes, neutrophils, and macrophages. LSP1 is a substrate for mitogen-activated protein kinase (MAPK)-activated protein kinase 2 and protein kinase C. The implication of LSP1 in diverse biological processes, such as inflammation and malignancy, suggests the major regulatory role of LSP1 in signal transduction(PMID: 38254711).