LSR Recombinant monoclonal antibody, PBS Only (Capture/Detector)

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• LSR Recombinant monoclonal antibody, PBS Only (Capture/Detector) (88350-2-PBS)

LSR Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 88350-2-PBS Copied!

Clone No. 260839G10

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

ILDR3, Lipolysis-stimulated lipoprotein receptor, LISCH, LISCH7

• 

  WB analysis using 88350-2-RR (same clone as 88350-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88350-2-RR (LSR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88350-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP03491-2

  The mean LSR concentration was determined to be 116.18 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load, 6.22 ng/mL in THP-1 cell extract based on a 1.20 mg/mL extract load and 4.82 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP03491-2

  Sandwich ELISA standard curve of MP03491-2, Human LSR Recombinant Matched Antibody Pair - PBS only. 88350-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12961. 88350-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array sample test of MP03491-1

  The mean LSR concentration was determined to be 1600.1 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 852.5 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, THP-1 cell extract were served as a negative control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array standard curve of MP03491-1

  Cytometric bead array standard curve of MP03491-1, LSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88350-3-PBS. Detection antibody: 88350-2-PBS. Standard: Ag12961. Range: 0.781-100 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88350-2-PBS targets LSR as part of a matched antibody pair:

MP03491-1: 88350-3-PBS capture and 88350-2-PBS detection (validated in Cytometric bead array)

MP03491-2: 88350-2-PBS capture and 88350-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12961

  Product name: Recombinant human LSR protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 339-589 aa of BC004381

  Sequence: SMRVLYYMEKELANFDPSRPGPPSGRVERAMSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRRSRAYMPPRSRSRDDLYDQDDSRDFPRSRDPHYDDFRSRERPPADPRSHHHRTRDPRDNGSRSGDLPYDGRLLEEAVRKKGSEERRRPHKEEEEEAYYPPAPPPYSETDSQASRERRLKKNLALSRESLVV

• Full Name: lipolysis stimulated lipoprotein receptor
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC004381
• Gene Symbol: LSR
• Gene ID (NCBI): 51599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86X29
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).